Pengaruh Pengunaan Probiotik Lactobacillus brevis dan Prebiotik Oligosakarida (Fructooligosakarida-Galaktoologosakarida) Terhadap Gambaran Darah Patin Siam (Pangasionodon Hypophtalmus) yang Diinfeksi Aeromonas Hydrophila

Yuke Eliyani
Widanarni Widanarni
Dinamella Wahjuningrum

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian probiotik Lactobacillus brevis, prebiotik oligosakarida (Fructooligosakarida- Galaktooligosakarida) terhadap nilai hemoglobin, hematokrit serta diferensial leukosit patin siam (Pangasionodon hypophthalmus) yang diinfeksi bakteri Aeromonas hydrophila. Uji yang dilakukan meliputi persiapan bakteri probiotik dan A.hydrophila, analisis prebiotik (oligosakarida), uji in vitro, dan uji in vivo. Parameter uji yang diamati selama penelitian adalah hemoglobin, hematokrit, serta diferensial leukosit. Penelitian terdiri dari lima perlakuan dengan masing-masing tiga ulangan, yaitu kontrol (+), kontrol (-), pemberian probiotik (Pro), pemberian prebiotik (Pre), pemberian probiotik ditambah prebiotik (Sin). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor. Data dianalisis dengan sidik ragam (ANOVA) pada selang kepercayaan 95%. Perbedaan antar perlakuan dilanjutkan dengan uji Duncan menggunakan program Xl-stat. Hasil pengamatan pada parameter hemoglobin, hematokrit, serta diferensial leukosit, berbeda nyata (p<0.05) dengan kontrol pada beberapa waktu pengamatan.

Keywords

probiotik; prebiotik; sinbiotik; Lactobacillus brevis; Aeromonas hydrophila; Pangasionodon hypophthalmus

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References

Anderson DP and Siwicki AK. 1993. Basic hematology and serology for fish health programs. Paper presented in second symposium on diseases in Asean Aquaculture “Aquatic Animal Health and The Environment”. Phuket, Thailand. 25 – 29th October 1993. 17hlm.

Bauer AW, Kirby WMM, Sherris JC, and Turck M. 1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol 36:493-496. Bernardeau M, Vernoux JP, Dubernet

SH and Guéguen M. 2008. Safety assessment of dairy microorganisms: The Lactobacillus genus. Food Microbiology 126: 278-285.

Blaxhall PC and Daisley KW. 1973. Routine haematologycal methods for use with fish blood. J. Fish Biology 5: 577 – 581.

Bratawidjaja KG. 2006. Imunologi Dasar. Edisi keenam. Balai Penerbitan Fakultas Kedokteran Universitas Indonesia. Jakarta.

Fraser TWK, Rønneseth A, Haugland GT, Fjelldal PG, Mayer I and H.I. Wergeland HI. 2012. The effect of triploidy and vaccination on neutrophils and B-cells in the peripheral blood and head kidney of 0+ and 1+ Atlantic salmon (Salmo salar L.) post-smolts. Fish & Shellfish Immunology 33: 60- 66.

Gropper SS, Jack LS and James LG. 2009. Advanced nutrition and human metabolism. 5thEd. Pre- Press PMG. Canada.

Harikrishnan R, Balasundaram C and Heo M.-S. 2012. Effect of Inonotus obliquus enriched diet on hematology, immune response, anddisease protection in kelp grouper, Epinephelus bruneus against Vibrio harveyi. Aquaculture, 344–349: 48–53.

Hernandez OH, Muthaiyan A, Moreno FJ, Montilla A, M.L. Sanz ML and Ricke SC. 2012. Effect of prebiotik carbohydrates on the growth and tolerance of Lactobacillus. Food Microbiology 30: 355 – 361.

Iwama G. 1996. The fish immune system. Academic press, San Diego-London-Boston-New York-Sydney-Tokyo-Toronto. 68-95, 185-222 p.

Lv-yun Zhu, Li Nie, Guan Zhu, Li-xin Xiang, Jian-zhong Shao. 2013. Advances in research of fish immune-relevant genes: A comparative overview of innate and adaptive immunity in teleosts. Developmental & Comparative Immunology 39:39–62

Mathious AS, Gatesoupe FJ, Hervi M, Metailler R and Ollevier F.. 2006. Effect of dietary inulin and oligosaccharides as prebiotiks for weaning turbot, Psettanational maxima. J. Aquaculture International 143: 219 – 229.

Merrifield DL, Dimitroglou A, Foey A, Davies SJ, Baker RTM, Bøgwald J, Castex M and Ringø E. 2010. Review: The Current status and future focus of probiotic and prebiotik applications for salmonids. Aquaculture 302: 1–18.

Putra AN. 2010. Aplikasi probiotik, prebiotik dan sinbiotik untuk meningkatkan kinerja pertumbuhan ikan nila (Oreochromis niloticus). Tesis. Bogor. Sekolah Pascasarjana, Institut Pertanian Bogor.

Rey A, Verján N, Ferguson HW and Iregui C. 2009. Pathogenesis of Aeromonas hydrophila strain KJ99 infection and its extracellular products in two species of fish. Veterinary Record 164: 493-499. Wartono Hadie, Lies Emmawati, Angela Mariana Lusiastuti. 2010. Peningkatan produksi benih ikan patin di unit pembenihan rakyat (UPR) melalui penerapan kekebalan bawaan (Maternal immunity). Prosiding forum inovasi teknologi akuakultur. Pusat Riset Perikanan Budidaya. Wedemeyer GA and Yasutake WT. 1977. Clinical methods for the assessment of the effect environment stress on fish health. Technical papers of the US.fish and wildfield services. US. Depart. of the interior fish and wildlife service 89: 1-17. Ziskowski J, Mercaldo-Allen R, Pereira JJ, Kuropat C, Goldberg R. 2008. The effects of fin rot disease and sampling method on blood chemistry and hematocrit measurements of winter flounder,Pseudopleuronectes americanus from New Haven Harbor (1987–1990). Marine Pollution Bulletin 56:740–750.

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